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Barplots showing percentage of viability of S9 epithelial cells following administration of different concentrations of herbicolin A (HrbA) and B (HrbB) (1: 0.5, 2: 2.5, 3: 10, and 4: 50 μg/mL). NC: negative control represents no treatment. PC: Positive control represents 1% Triton X. Box-and-whisker plots show the median (horizontal line), interquartile range (box), and minimum and maximum values (whiskers). Data are shown as individual values with group means. Statistical significance was assessed using one-way ANOVA followed by Holm-corrected pairwise Student’s t -tests. The equation shown at the top of the plot reveals the statistical model used for comparisons. The values shown at the bottom of the plot indicate the ANOVA results, including the F-statistic with degrees of freedom, the associated p-value, and the effect size with 95% confidence interval.

Journal: bioRxiv

Article Title: Herbicolin A, an antifungal lipopeptide produced by Pantoea agglomerans APC 4211 is a promising biocontrol agent against food spoilage fungi

doi: 10.64898/2026.05.21.726617

Figure Lengend Snippet: Barplots showing percentage of viability of S9 epithelial cells following administration of different concentrations of herbicolin A (HrbA) and B (HrbB) (1: 0.5, 2: 2.5, 3: 10, and 4: 50 μg/mL). NC: negative control represents no treatment. PC: Positive control represents 1% Triton X. Box-and-whisker plots show the median (horizontal line), interquartile range (box), and minimum and maximum values (whiskers). Data are shown as individual values with group means. Statistical significance was assessed using one-way ANOVA followed by Holm-corrected pairwise Student’s t -tests. The equation shown at the top of the plot reveals the statistical model used for comparisons. The values shown at the bottom of the plot indicate the ANOVA results, including the F-statistic with degrees of freedom, the associated p-value, and the effect size with 95% confidence interval.

Article Snippet: Human airway epithelial cells (S9) were obtained from the American Type Culture Collection (LGC Promochem, Wesel, Germany) (no. CRL-2778).

Techniques: Negative Control, Positive Control, Whisker Assay